Application

Research Sub CategoryCytoskeleton

Western blotting: 1:100-1:1,000; nuclear preparations recommended; detects 67-68kDa lamin B2 protein

Immunocytochemistry on fixed cells (methanol/acetone fixation): 1:100-1:200.Flow cytometry: 1:100-1:200.

Optimal working dilutions must be determined by the end user.

Research CategoryCell Structure

This Anti-Lamin B2 Antibody, clone LN43 is validated for use in FC, WB, IH for the detection of Lamin B2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Immunogen

Detergent insoluble fraction of potoroo cell line PtK1

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified culture supernatant. Liquid in PBS containing 0.09% sodium azide.

Format: Purified

Specificity

Lamin B2. Recognizes an epitope located in the C-terminal part of lamin B2.

Storage and Stability

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.